Medical Mycology: Open Access

Chemical stressors increase polysaccharide secretion in Lentinus squarrosulus

2^nd International Conference on Mycology & Mushrooms
September 25-26, 2017 Chicago, USA

Anike F N, Isikhuemhen O S, Obinyan B and Okhuoya J A

North Carolina A&T State University, USA
University of Benin, Nigeria

Posters & Accepted Abstracts: Med Mycol Open Access

DOI: 10.21767/2471-8521-C1-003

Abstract

Introduction: Lentinus squarrosulus is a seasonal mushroom consumed by millions of people. Beyond nutritive factors, L. squarrosulus elicit medicinal benefits through its polysaccharide content. While attempts at domestication and mass production of L. squarrosulus (on going) is one way to increase polysaccharide output, mechanisms that increase polysaccharide secretion in vitro is an attractive alternative both for process optimization and product recovery. The study evaluated the effectiveness of chemical stressors such as acidity and sodium chloride in increasing polysaccharide secretion in L. squarrosulus. Methodology: L. squarrosulus was cultured in submerged fermentation under different concentrations of sodium chloride (0.5, 1, and 2 g/l) and pH levels (3, 4, 5, 6, 7, 8, and 9) including their controls. The basal medium was composed of soluble starch (10 g/L), Dextrose (5 g/L) and yeast extract (2 g/L). Five (5) replicates of each medium were inoculated with 5 mm agar blocks from 3-day old L. squarrosulus culture and incubated at 28Ã?Â?C for 14 days. Mycelia were harvested by filtration and polysaccharide precipitated from supernatant in 3 volume ethanol overnight, centrifuged, dried and weighed. Result: The two chemical stressors increased secretion of polysaccharides in L. squarrosulus tremendously compared to non-stressed cultures. Acidic pH was more favorable to polysaccharide secretion than alkaline pH. Highest polysaccharide (0.026g) was detected in pH 3 and least (0.01g) in pH 6-9. Low sodium chloride concentration (0.5 g/L) resulted in more polysaccharide secretion while higher salt concentrations (2 and 3 g/L) inhibited growth completely. Acidic medium was more effective in inducing polysaccharide than low salt medium (0.26 g vs 0.2 g). Conclusion: Chemical stress using sodium chloride and acidic pH induced higher polysaccharide secretion in L. squarrosulus. The method could be optimized and adapted for commercial production of L. squarrosulus polysaccharide.

Biography

Anike F N is an experienced Researcher and Educator with expertise in mushroom and fungal biotechnology. She conducts research and trains students in this field of study, authored and co-authored many peer reviewed journals and publications.