Nano Research & Applications

Biosynthesis of selenium nanoparticles by aspergillus flavus and candida albicans for antifungal applications

38^th International Conference on Advanced Nanotechnology
August 01-02, 2022 | WEBINAR

Mohammadali Ghanbarinahzagh

Department of Medical Parasitology and Mycology, Ghaem hospital, Mashhad University of Medical Sciences, Mashhad, Iran

Posters & Accepted Abstracts: Nano Res Appl(Los Angeles)

Abstract

Biosynthesis of nanoparticles can replace the available chemical and physical methods by offering new procedures as green syntheses that have proved to be simple, biocompatible, safe, and cost-effective. Recently, antifungal resistance has been reported against different species of Aspergillus and Candida opportunistic fungi. Selenium nanoparticles (Se-NPs) were biosynthesized using standard strains of Aspergillus flavus and Candida albicans. The presence of nanoparticles was confirmed by UV-Vis, FT-IR, FESEM, EDX, XRD, and Zeta potential. Common fungal strains were cultured in Sabouraud dextrose agar medium to perform the sensitivity test based on the minimum inhibitory concentration (MIC) method in duplicate. The utilization of Se-NPs at concentrations of 1, 0.5, and 0.25 μg/ ml or in some strains even more minor than 0.125 μg/ ml resulted in zero growth of fungal agents. However, antifungal drugs inhibited their growth at concentrations of 2, 4, 8, 16, and 64 μg/ ml itraconazole (ITC). Also, MIC breakpoints for amphotericin B (AMB) and anidulafungin (AFG) were 2 μg/ ml for defining resistance in some isolates. Based on the obtained results, biological NPs produced by Aspergillus and Candida at different concentrations exhibited favorable inhibitory effects on the growth of fungal strains. Keywords: Biosynthesis; Selenium nanoparticles; Antifungal resistance; MIC; Aspergillus; Candida