Journal of Transmitted Diseases and Immunity

Apoptosis of immune cells in type 1 diabetes mellitus

Joint Event on 22^nd Edition of International Conference on Immunology and Evolution of Infectious Diseases & 12^th Edition of International Conference on Tissue Engineering and Regenerative Medicine
May 10-11, 2018 Frankfurt, Germany

Hovsepyan L M, Kazaryan G S, Zanginyan A V, Akopdzhanyan A A, Zakharyan G V, and Petrek M

Institute of Molecular Biology - NAS, Republic of Armenia Palacky University, Czech Republic

Posters & Accepted Abstracts: J Transm Dis Immun

DOI: 10.21767/2573-0320-C2-006

Abstract

Type 1 diabetic mellitus (T1DM) is known to be associated with progressive destruction of �?²-cells of the pancreas. Dysregulated immunity and programmed cell death are an important link in pathogenesis of diabetes. In this study, we examined expression levels of interleukin-2, BCL1, ANXA-11 genes in patients with T1DM. The study was done with blood leukocytes of 30 T1DM patients and 70 healthy controls. Reversetranscription PCR was done with Transcriptor first strand cDNA synthesis kit from Roche Life Science. The study of IL-2 and BCL1 gene expression level in peripheral blood leukocytes indicated that the median gene expression levels of IL-2 and BCL1 are increased in patients with T1DM patients compared with control group of healthy persons. The study of apoptosis by annexin test has revealed an increased level of ANXA-11 expression in T1DM patients. The obtained data can serve as an additional source for understanding the pathogenesis of T1DM mechanisms. hzang@mail.ru