American Journal of Ethnomedicine

Anti-inflammatory effects of phenolic compounds from Quercus mongolica on UVB-irradiated human skin cells

6^th Edition of International Conference on Pharmacognosy and Medicinal Plants
April 16-17, 2018 Amsterdam, Netherlands

Min Won Lee and Han Hyuk Kim

Chung-Ang University, South Korea

Posters & Accepted Abstracts: Am J Ethnomed

DOI: 10.21767/2348-9502-C1-006

Abstract

Quercus mongolica (QM) is a species of Quercus native to Eastern Mongolia, Siberia, China, Japan, and Korea. Species of Quercus have been used as an oriental traditional medicine in north-east Asia for the treatment of inflammation of the oral, genital, or anal mucosa, and externally for inflammation of the skin. Previous studies on the chemical composition of Quercus species have led to the isolation of various triterpenoids, flavonoids, and phenol glucoside gallates exhibiting a variety of bioactivities including gastrointestinal disorders, anti-bacterial and anti-oxidative activities. Previously, we conducted isolation and elucidation of the structures of the known to compounds from QM including one ellagitannin [pedunculagin (PC)], five flavanoids [(+)-gallocatechin, (+)-catechin, quercetin-3-O-(6”- O-galloyl)-β-D-glucopyranoside (QGG), kaempferol-3-O-β-Dglucopyranoside- 7-O-α-L-rhamnopyranoside, kaempferol-3-O- (6’’-galloyl)-β-D-glucopyranoside]. In this work, we measured inhibitory activities on chemokine and cytokine production of the extracts and compounds isolated from QM. The activities of QM and its compounds against MCP-1, TARC, IL-6, IL-8, IL-10 and IL-13 in keratinocytes irradiated with UVB showed that EtOAc fraction and PC and QGG showed the best activities. Based on the inhibitory activities of cytokines and chemokines, PC and QGG were selected as candidate the treatment of chronic skin diseases and evaluated their protein and mRNA levels of inflammatory mediators including COX-2, PGE2, cytokines and chemokines in UVBâ�?irradiated HaCaT cells and also quantified by western blotting and RT-PCR. PC and QGG diminished UVB-irradiated protein level expression of COX-2 and PGE2, downstream products in dosedependent manners. These results suggest that PC and QGG are potential anti-inflammatory for treating inflammation of skin. Recent Publications 1. Thi Tam Le and Min Won Lee, et al. (2017) Anti- Inflammatory and anti-oxidative activities of phenolic compounds from Alnus sibirica stems fermented by Lactobacillus plantarum subsp. argentoratensis. Molecuels 22(9):1566. 2. Sung Hye Youn, Min Won Lee, et al. (2017) Anti- Inflammatory and Anti-urolithiasis effects of polyphenolic compounds from Quercus gilva Blume. Molecules 22(7):1121. 3. Manh Heun Kim, Min Won Lee, et al. (2016) Two new phenolic compounds from the leaves of Alnus sibirica Fisch. ex Turcz. Natural Product Research 30(2):206– 213. 4. Jun Yin, Min Won Lee (2016) Inhibitory activities of phenolic compounds isolated from Adina rubella leaves against 5α-reductase associated with benign prostatic hypertrophy. Molecules 21(7):887. 5. Han Hyuk Kim, Min Won Lee, et al. (2015) Inhibition of matrix metalloproteinase-1 and type-I procollagen expression by phenolic compounds isolated from the leaves of Quercus mongolica in ultraviolet-irradiated human fibroblast cells. Archives of Pharmacal Research 38(1):11–7.

Biography

Min Won Lee has his expertise in Pharmacognosy and Natural Product Derived Medicine. He is a Professor at the College of Pharmacy, Chung-Ang University and served as a President in the Korea Society of Pharmacognosy in 2017. He has extensive experience in working on phytochemical constituents from natural herbal medicine and finding effective compounds to treat various kinds of chronic inflammatory diseases including atopic dermatitis and benign prostatic hyperplasia. Because of these successful biological activities, the isolated compounds and extract of natural herbal medicine were registered on several patents in Korea and in US and China. Many papers using these results including effective compounds isolated from natural plants have been published in famous international journals.

E-mail: mwlee@cau.ac.kr