Abstract

Cell culture has become an indispensable technology in many branches of the life science. It provides the basis for studying the regulation of cell proliferation, differentiation & product form at ion in carefully controlled conditions with processes & analytical tools which are scalable from the level of single cell to in excess of 10kg wet weight of cells. The cell culture has provided the entire human genome, and to dissect the pathway soft intracellular & intercellular signalling which ultimately regulates gene expression. As roller bottle culture is considered the first scale up step for choragic dependent cells from stationary flasks or bottle(Langdonetal2004). This achieved by using all internal surfaces for cell growth, rather than just the bottom of the bottle. The added advantages are that a smaller volume of medium & thus a higher product titre can be achieved the cells are more efficiently oxygenated due to alternative exposure  to medium and gas phase &dynamic systems usually generate higher unit cell densities than stationary systems.