In Forensic Laboratory Rajasthan, India different types of cases are received in Toxicology Section where the visceral content comprising of Piece of Stomach, Intestine, Liver, Spleen, Kidney, Lungs, Heart, Brain, Blood sample, Urine Sample, Skin piece (injection site or site of Bite) etc is being received for examination. All types of poisons is grouped into metallic poisons, ethyl - methyl alcohol, cyanide, alkaloids, barbiturates, tranquillizers and insecticides are often checked. By far if we go on to see the statistics data In India most of the poisoning cases found positive for Aluminium phosphide and Insecticides, Some cases are of drugs poisoning where the drugs are being used for suicide, murder purpose or due accidental reasons viz. negligence use of drugs during treatment by medical doctors. A scientist has to think about all related aspect along-with analysis to assist the cases to reach at concrete result that help get justice. The wide range of cases of poisoning specifies from cyanide, nitrite, alcohol, insecticide, carbon-monoxide, phosphine, snake venom, medicinal drugs for different purposes Robbery, Murder, Homicide, Suicide etc are quite common. After performing separation and extraction methodologies separation of organic and inorganic poisons took place. The confirmation of poison was done using GC-MS-MS, LC-MS-MS and UV-Visible Spectrometry for organic poisons. For inorganic poisons the use of Inductively Coupled Plasma with Mass Spectrophotometer and EDX-8000 and Ion chromatography techniques are proved highly significant. EDX-8000 technique is useful in detection of Gun Shot residues in traces. Several cases are sorted out of it and establishing the presence of gunshot residues on the hands of criminals solves typical mysteries. Latest changing scenario of crime needs up-gradation in techniques so that they can be used to establish strong evidences in Court of Law