Abstract

The treatment of bone defect has always been difficult in clinical practice, usually aassociated with infection and angiogenesis disorders, which further affect bone healing. Therefore, it is important to develop a suitable sized scaffold that can accelerate both osteogenesis and angiogenesis for the treatment of bone defects. Herein, we synthesized a multifunctional coating (QCS- GO-PDA) composed of quaternized chitosan (QCS), graphene oxide (GO) and polydopamine (PDA), and used selective laser sintering (SLS) in 3D printing to form a composite of polylactic acid/hydroxyapatite (PLA/HA), and then applied the multifunctional coating to the PLA/HA scaffold. Compared with the single component, the antimicrobial osteogenic effect was enhanced after covalently grafted with the QCS and GO, and the PDA provided adhesion and contributed to the surface functionalized modification of the 3D printed scaffold. The results show that the combination of the multifunctional coating and the 3D printed personalized scaffold (PLA/HA^QCS-GO-PDA) can significantly promote the regeneration and repair of bone tissue. In vivo and in vitro experiments showed that the PLA/HA^{QCS-GO- PDA} group increased the osteogenic ability of the bone defect during healing, effectively inhibited the production of inflammatory factors in the later period, and induced bone regeneration, which is considered as a novel type of bone regeneration scaffolds with potential for clinical application.