Abstract

The emergence of carbapenem-resistance among Enterobacteriaceae has been increasingly reported worldwide. In Songklanagarind hospital, carbapenem-resistant Klebsiella pnuemoniae (CRKP) are the highest prevalence among other CRE species. In this study, we aimed to investigate the incorporation of nanotechnology with the existing therapeutics to combat antimicrobial resistance. Herein, silver nanoparticles (AgNPs) was
synthesized using durian peel extract as the reducing agents. The transmission electron microscope (TEM) and dynamic light scattering (DLS) measurement revealed small grains of AgNPs in spherical shapes with sizes of ~20-30 nm. The antibacterial activities of the green-synthesized AgNPs alone and in combination with carbapenems have been evaluated against K. pneumoniae ATCC®700603TM and CRKP clinical strains by broth
microdilution assay, time-kill assay and checkerboard assay