Abstract

The present study developed an optical biosensor based on Carbon Dots (CDots) obtained from Elaeis guineensis biochar, for the detection of a model molecule, in this case, Bovine albumin serum (BSA). The CDots had a size of 2.5 ± 0.7nm determined through a High-Resolution Transmission Electronical Microscopy (HR-TEM), a Z potential of -56.1 ± 2.37 mV and a Quantum yield of 1.69%, with emission and excitation wavelengths of 428 and 320 nm, respectively. As a bioreceptor molecule, the anti-BSA was used and conjugated to the CDots surface using the carbodiimide method. The fluorescence of the CDots was evaluated through a fluorescence spectroscopy and posteriorly modulated using graphene oxide. Finally, the performance of the biosensor was analysed by evaluating the specificity and sensitivity, where a qualitative biosensor was obtained that detects in a range of 1000 - 10 mg / μL of BSA. With the implementation of this technology, it will contribute to the improvement of the quality of life of Colombian vulnerable populations while contributing to the closing of cycles of the oil palm industry