Whartons Jelly Stromal Cells treated with diseased liver serum enhance angiogenesis and reduced apoptosis

Liver fibrosis is the excessive accumulation of ECM that destroys the hepatic architecture by making fibrotic scars or wounds in liver. In response to fibrosis different cytokines and growth factors can direct the migration of bone marrow mesenchymal stromal cells (BMSCs) towards wounded liver that could heal the scar of liver. Wharton's jelly (WJ) is a well-known source of stromal cells (SCs). A huge amount of stromal cells can be isolated from umbilical cord’s Wharton's jelly (MJSCs). This study uses the concept of cell migration under the influence of serum derived factors to be used for regeneration. Thus, in the present study, different concentrations of blood serum from liver fibrosis patients (injured serum) and normal subjects (normal serum) was used for the preconditioning of 2nd passage WJSCs to explore their potential for the wound healing via improved angiogenesis, anti-oxidative enzymes and reduced injury. Results indicate improved survival and wound healing ability of the 5% serum treated WJSCs. 5% serum treatment have also shown increased angiogenesis and reduced H2O2 injury levels as evidenced by gene expression analysis. Therefore, this study would aid healing of wound via improved angiogenesis and reduced injury levels investigated in in-vitro studies.

Author(s): Sana Javaid Awan

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