Validation of Abortifacient Potential of Siddha Polyherbal Formulation Maavilingathy Mathirai in Female Wistar Albino Rats

Objective: To study the abortifacient potential of the Siddha polyherbal formulation Maavilingathy Mathirai in Wistar Albino rats.
Materials and Methods: Male and Female albino rats of Wistar strain weighing about 120-200 gm in the ratio of 1:2 were caged. Pregnancy was confirmed and the rats were divided into three groups of six animals each and the rats were administered the drug through gastric gavage from 6th to 15th day of pregnancy. The 3 groups of animals were administered 2ml/kg of CMC, 100 mg/kg, 200mg/kg of MLM for 10 days. The animals were laparotomised on 19th day of pregnancy and the uterine horns were subjected for the examination of the live and dead fetuses, implantation sites, resorptions. The serum was separated and let down into a vial and then and subjected to ELISA method for the assessment for LH, Estradiol, FSH and Progesterone.
Results and Discussion: The MLM 100 and MLM 200 reduced the no. of implants in the uterus significantly. The percentage of abortion calculated revealed that it was 34% at the dose level of 100mg/kg and 69% at the dose of 200mg/kg and a significant abortifacient effect indicating the reduction of the number of viable fetuses.FSH hormone level is highly significant (p<0.01) at both the dose levels of MLM100, 200mg/kg. The results showed significant decrease in LH at a dose level 0f 100mg/kg and highly significant decrease at the dose level of MLM200mg/kg. Estradiol hormone is highly significant (p<0.01) at both the dose levels of MLM100, 200mg/kg. Progesterone hormone is also highly significant (p<0.01) at both the dose levels of MLM100, 200mg/kg. The reduction in the level of hormones proved to be the prime reason for abortifacient activity.

Author(s): B. Sathya, V. Velpandian, M. Pitchiah Kumar, P. Thenmozhi, S. Vidhya and V. Banumathi

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