Validated RP-HPLC method for the determination of Benzoic acid in bulk and pharmaceutical formulation

A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Benzoic acid in Bulk and Pharmaceutical Formulation. Isocratic elution at a flow rate of 1.0 ml/min was employed on symmetry C18 5 μm (4.6 x 150 mm) column at ambient temperature. The mobile phase consisted of Acetonitrile: Buffer in the ratio of 45:55 v/v. The UV detection wavelength was 254nm and 20μl sample was injected. The retention time for Benzoic acid was ± 18 min. The percentage RSD for precision and accuracy of the method was calculated. The method was validated as per the ICH guidelines. The method was successfully applied for routine analysis of Benzoic acid in the rapid and reliable determination of Benzoic acid in Pharmaceutical formulation.

Author(s): M. Madhu, V. Sreeram, A. V. D. Nagendrakumar and T. V. Reddy

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