UV spectrophotometric estimation of ofloxacin by area under curve methods in bulk and pharmaceutical dosage form

Simple and precise UV spectrophotometric method by area under curve [AUC] - have been developed and validated for the estimation of ofloxacin in bulk and its tablet formulation. The standard and sample solutions of ofloxacin were prepared in 0.1 N hydrochloric acid. Ofloxacin was estimated area under curve (AUC) method the zero order spectrum of ofloxacin was measured in between 288 nm to 298 nm. Beer’s law was obeyed in the concentration range of 1 to 10 µg / ml with coefficient of correlation value 0.9995. These methods were tested and validated for various parameters according to ICH guidelines. The precision expressed as relative standard deviation were of 0.03723% method respectively. The proposed method was successfully applied for the determination of ofloxacin in pharmaceutical formulation. Results of the analysis were validated statistically and were found to be satisfactory. The proposed method is simple, easy to apply, low-cost and require relatively inexpensive instruments.

Author(s): Rajan V. Rele

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