UV spectrophotometric determination of Darifenacin Hydrobromide in bulk and pharmaceutical dosage forms

A simple and sensitive ultra-violet spectrophotometric method was developed for the estimation of darifenacin hydrobromide in bulk and pharmaceutical dosage forms. This method is based on darifenacin hydrobromide, showing absorbance at 286 nm in methanol. This method obeys Beer’s law in the concentration range of 10 to100 μg ml^-1. The proposed method is precise, accurate and reproducible and can be extended for the analysis of darifenacin hydrobromide in bulk and tablet formulations. The % recovery is greater than 98 %, hence showed that the method was free from the interference of excepients. The mean % recovery ranges from 99.186 ± 0.9627 % to 100.914 ± 0.9648 %. Intraday precision had a % RSD of 0.004939 and for that of intermediate precision the % RSD is found to be 0.636. The value of standard deviation and % R.S.D were found to be less than 2 which shows that the method is highly precise. The % RSD for analysis is found to be around 99.69 ± 0.909. The solutions were found to be stable for the various concentrations ranging from 10-100% at 30 and 60 minutes.

Author(s): NK Sathish, Pradeep V. Khandelwal, IA Chethan and Ashwinee Kumar Shrestha

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