Symptomatology and Molecular Variations of Graminicolous Downy Mildew Pathogens in Hybrids and Inbreds of Maize

This study was performed to identify Peronosclerospora species found in Tamil Nadu based on sequence analysis of the COX-2 gene. In addition, sequence data in total, 25 in breeds infected with isolates of Peronosclerospora were investigated in this study. They were obtained from gene bank accessions of maize research station, Vagarai, Tamil Nadu and raised in Kharif and Rabi seasons. Both infected seeds and leaves were tested for the DNA analysis. Sequence analysis of COX-2 and phylogenetic inference were performed on 5 isolates. A set of primers developed in this study, PCOX2F and PCOX2R, was used for PCR amplification. Phylogenetic analyses showed that 5 isolates were divided into three groups. Maize crop is infected with 3 species of Pernosclerospora in two different growing seasons.

Author(s): Radhajeyalakshmi R*, Harish S and Karthikeyan G

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