Studies on the in vitro propagation of Calamus travancoricus

In vitro propagation techniques were applied for the mass propagation of economically important plant Calamus travancoricus Bedd.ex. Becc. & Hk.f. For callus induction, the explants were cultured on MS medium augmented with different auxins separately. The study showed 2, 4-D (10mg/ml) is effective to induce callus. The calli were subcultured on MS medium with various concentrations of cytokinins for shoot formation. The medium supplemented with BAP (5mg/l) showed better response. The medium supplemented with1.0mg/l of GA3 was found suitable to culture microshoots for elongation. The in vitro shoots thus obtained were successfully rooted in ½ MS medium supplemented with IBA (1.5mg/l) and NAA (1mg/l) separately. The rooted plantlets were transferred to the field after acclimatization. Synthetic seeds were prepared.

Author(s): H. N. Krishna Kumar*, S. D. Preethi and Jyoti Bala Chauhan

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