Statistical optimization and characterization of prepared Fluconazole topical liposomal gel for improved skin permeation

The aim of the study is to prepare topical liposome of fluconazole by standard lipid film hydration method followed by incorporation into freshly prepared hydrogel for effective topical permeability. Different ratio of cholesterol and phospholipid was considered as a lipid carrier and 23 factorial design was used to evaluate the influence of different conditions on entrapment efficiency and drug release from liposomal gel. Prepared fluconazole liposomal gel was characterized by photo microscopic study, entrapment efficiency, drug content, stability studies, surface topography and in-vitro release study. DSC and FTIR analysis were performed to characterize the state of drug and lipid modification shape and surface morphology were determined by SEM which revealed white spherical shape of the formulation. The consequence of simultaneously varying two preparation factors that is Ratio of Phospholipid: Cholesterol and Hydration volume and their interaction on response were modeled by response surface methodology (RSM). Three-dimensional response surface plots and mathematical polynomial equations were used to correlate the index and control. In vitro dissolution study shows that R2 value of formulation F9 was found to be 0.998 which is significant amongst all with a zero order drug release profile. The final optimized formulation having the composition of PC was 84.22 mg with cholesterol 20 mg and hydration volume 13.83 ml incorporated in 2% carbopol gel.

Author(s): Subhabrota Majumdar

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