Spectrophotometric Estimation of Ferulic Acid from Ferula asafoetida by Folin - Ciocalteu’s Reagent

A simple, sensitive and reproducible spectrophotometric method has been developed by means of Folin-ciocalteu’s reagent in 15 % sodium carbonate for quantitative estimation of ferulic acid from Ferula asafoetida. The blue coloured chromogen thus formed after the reaction was measured at wavelength of maximum absorption 718 nm for ferulic acid against the blank reagent. The chromogen obeyed linearity over the range of 1μg/ml - 8μg/ml. The method was further validated as per ICH guidelines by studying various parameters such as linearity, precision, accuracy, limit of detection and limit of quantification.

Author(s): Aruna P Jadhav, Joseph A Kareparamban, Pravin H Nikam, Vilasrao J Kadam

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