Simultaneous Determination of Fluconazole and Tinidazole in Combined Dose Tablet using High Performance Thin Layer Chromatography

This paper presents an HPTLC method, validated for simultaneous determination of fluconazole (FLZ) and tinidazole (TNZ) in a combined dose tablet. Chromatography was performed on silica gel 60F₂₅₄ plates with 250 μm thickness, using Toluene: Ethyl acetate: Chloroform: Methanol (1.2:3:2:0.8 v/v) as a mobile phase. For the proposed method, linearity (FLZ-r² > 0.996; TNZ-r² > 0.997), sensitivity (FLZ-3.04 ng/spot; TNZ-11.12 ng/spot), recovery (FLZ- 98.66%-99.13%; TNZ- 99.26%-100.54%), and repeatability were found to be satisfactory. The HPTLC method offers a simple, sensitive, rapid and cost effective technique that can be applied successfully in routine quality control of formulations containing FLZ and TNZ.

Author(s): Dhananjay B. Meshram, Priyanka Mishra, Sapna D. Desai, Madhukar R. Tajne

Abstract | Full-Text | PDF

Share This Article