Reversed phase ultra performance liquid chromatography method for determination of olopatadine hydrochloride from active pharmaceutical dosage form

A simple, rapid and accurate ultra performance liquid chromatography method is described for determination of olopatadine hydrochloride from active pharmaceutical ingredients. The separation of drug was achieved on Acquity BEH C18 (50 X 2.1 mm) 1.7μ column. The mobile phase consisted of a mixture of buffer and mobile phase B (70:30 % v/v acetonitrile and methanol). The buffer was mixtures of 0.01 M ammonium acetate adjusted the pH 4.5 with dilute acetic acid and the mobile phase B consisted a mixture of acetonitrile and methanol (70:30% v/v). The detection was carried out at wavelength 299 nm. The mixture of water and acetonitrile (50:50% v/v) was used as a diluent. The method was validated for system suitability, linearity, accuracy, precision, robustness, stability of sample solution. The method has been successfully used to analyze olopatadine hydrochloride from active pharmaceutical ingredients.

Author(s): Rajan V. Rele

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