Research on leaves of Micromelum pubescens and its phytochemicals, in-vitro Thrombolytic and in-vivo Antidepressant activity by methanol extract

Present study aims to open new avenues for the improvement of medicinal uses of Micromelum pubescens leaves under family of Rutaceae for the selected area for Phytochemical screening, in- vitro Thrombolytic assay, and in vivo Antidepressant properties of the methanolic extract. In-vitro thrombolytic activity of the plant extract is determined with streptokinase as a positive control & water as negative control. In-vivo antidepressant activity of plant extract is followed by force swim test & tail suspension test. After phytochemical screening of Micromelum pubescens, there is presence of carbohydrate, alkaloids, glycosides, tanins, phenol, terpenoids and saponins. In case of thrombolytic activity ,average lysis of the blood clot on the basis of this, % clot lysis = (Weight of the lysis clot / Weight of clot before lysis) × 100 was found 25.03%. In antidepressant test animals treated with two doses of plant extract 200 and 400 mg/kg. In case of tail suspension test, the plant extract showed decreases in their immobility times, which was significant 160second and 142.66second respectively when compared with control (204.33second).Similarly, animals treated with imipramine (10 mg/kg), as expected, showed a significant decrease in the immobility time (85.33second). In case of force swimming test, plant extract showed decreases in their immobility times, which was significant 161.33s(200 mg/kg) and 140s(400 mg/kg) when compared with control (191.33scond).Similarly, animals treated with imipramine (10 mg/kg), as expected, showed a significant decrease in the immobility time (92.66second). The result indicate that, Micromelum pubescens have possess moderate.

Author(s): Mohammad Jamshedur Rahman, Joy Chowdhury, Gula Jannat, Fahmida Haque, Sadiya Chowdhury, Shahriyer Mohammad Rafid, Md. Mobarak Hossain and Md. Shahidul Islam

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