Rapid RP-HPLC Method for Estimation of Zidovudine from Tablet Dosage Form

A new reverse phase HPLC method is developed for the rapid estimation of zidovudine, an antiviral agent from tablet dosage formulation. Standard solution of concentration 1 mg/mL was prepared in methanol. The analysis was carried out on a Nucleosil (4.6 mm I.D x 250 mm) C18 column using mobile phase Methanol. The flow rate was maintained at 1.0 ml/min. Detection was monitored at 267 nm. The retention time of drug was found to be 2.37 min. The validation of the proposed method was in terms of linearity and range, accuracy and precision. The drug showed the linear response over the concentration range from 400 to 1600 μg/mL. The percentage recovery obtained for drug from tablet formulation was 99.72 with relative standard deviation of less than 1%. Since the analysis is completed within 5 minute, the proposed method could be employed for routine analysis zidovudine from tablet formulation

Author(s): J.V. Manwar1, B.V. Sonawane2, S.V. Patil3 and S.P. Takle3

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