Rapid micropropagation and callus induction of Terminalia bellerica Roxb. -An endangered plant

An in vitro micropropagation system has been developed for Terminalia bellerica Roxb., an important Indian medicinal plant. Nodal segments obtained from 15-d-old aseptically grown seedlings were used as explants. MS medium containing 2.0 mg/l BAP was found most suitable for culture initiation. Although shoot multiplication was achieved on MS medium containing BAP and Kn, the maximum number of shoots was obtained with 3.5 mg/l BAP+ 0.5 mg/l Kn. Best rooting response was observed on medium containing quarter strength MS salts, 0.8% agar and 1.0 mg/l IBA. Plantlets were hardened initially in culture room conditions and then transferred to misthouse. Maximum callus induction response was observed on MS medium supplemented with 0.25 mg/l 2,4-D+ 0.3 mg/l Kn within 4 weeks from leaf petiole.

Author(s): Jitendra Mehta*, Monika Sain, Banwari Lal Mathuriya, Ritu Naruka, Ambika Kavia and Dev Ratan Sharma

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