Quantitative estimation of Gallic Acid, Rutin and Quercetin in certain herbal plants by hptlc method

An HPTLC method was developed for the quantitative estimation of gallic acid rutin and quercetin from aqueous and ethanolic extract Eruca sativa, precoated HPTLC silica gel 60 F254 as stationary phase and mobile phase for gallic acid Toluene: Ethyl Acetate: Formic Acid [7:5:1 v/v/v/v/v] and mobile phase for quercetin and rutin, ethyl acetate: glacial acetic acid: formic acid: water [100:11:11:25, v/v/v/v/v]. Detection and quantification were performed densitometrically at λ 280nm for gallic acid, 280 nm quercetin and 366nm for rutin. The standard Rf values of gallic acid, quercetin and rutin are 0.35±0.01, 0.98±0.01 and 0.34±0.02 respectively. The total peak areas of the standards [gallic acid, quercetin and rutin] and the corresponding peak areas of extracts were compared and the Gallic acid, quercetin and rutin content were estimated to be 356.1, 4591.0 and 1277.1

Author(s): Sajeeth C.I 1, Manna P.K2, R. Manavalan2, Jolly C.I3

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