Phytochemical and Chromatographic studies in the flowers of Woodfordia fruticosa (L) Kurz.

The present study primarily aims to carry out a preliminary phytochemical screening so as to detect the major class of compounds present. TLC profiling of the Woodfordia fruticosa flowers was carried out using sequential extracts of solvents with varying polaity; petroleum ether, chloroform and methanol respectively. The TLC documentation was done in short UV(254nm), long UV(365nm) and visible light after derivatisation with Anisaldehyde Sulphuric acid as the spray reagent.. Then DPPH free radical scavenging assay was carried out in the flowers so as to detect its antioxidant activity. The flower has a significant sweetness when tasted; so the total estimation for the starch content in the plant was carried out. The HPLC studies were performed in the methanolic extract of the plant, since it gave better separation than the other two solvents. The results obtained can be used for the genuine identification of the plant from its adulterants.

Author(s): A. Finose* and K.Devaki

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