Phytochemical analysis and antibacterial properties of leaf extract of Azima tetracantha (Lam.)

Azima tetracantha (Lam.) belonging to family Salvadoraceae was collected from Orathanadu, Tahnjavur District. The phytocompounds were separated by using TLC (Thin Layer Chromatography). Phytocompound analysis of Azima tetracantha revealed the presence of alkaloids, flavonoids and sterols. The isolated phytocompounds were used for antibacterial activity performed by using agar well diffusion method. Antibacterial activity of phytocompounds separation from alkaloids, flavonoids and sterols were tested against Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and Bacillus subtilis. The sterols compound exhibited maximum zone of inhibition against Bacillus subtilis (25mm), and Pseudomonas aeruginosa (24mm). The alkaloid compound showed minimum zone of inhibition was observed in Bacillus subtilis (12mm) and Pseudomonas aeruginosa (12mm). Maximum zone of inhibition was observed in sterols compound when compared with alkaloids and flavonoids.

Author(s): M. Gowthami, S. Tamil Selvi, G. Senthil Kumar and A. Panneerselvam

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