Pheophorbide a isolated from Gelidium amansii inhibits adipogenesis by down-regulating adipogenic transcription factors in 3T3-L1 adipocytes

Background: Adipocyte lipid accumulation causes adipocyte hypertrophy and adipose tissue increment, leading to obesity. Thus, this study investigated the anti-adipogenic effects of pheophorbide A isolated from Gelidium amansii in 3T3-L1 adipocytes. Methods: Upon differentiation of 3T3-L1 pre-adipocytes into adipocytes, they were treated with pheophorbide A. Results: Pheophorbide a inhibited triglyceride accumulation and stimulated glycerol release in a dose-dependent manner in 3T3- L1 adipocytes. In addition, pheophorbide A significantly decreased leptin levels in 3T3-L1 adipocytes. Pheophorbide A inhibited adipogenesis via suppression of the expression of adipogenic transcriptional factors including peroxisome proliferator-activated receptor enhancer binding protein sterol regulatory element binding protein 1c (SREBP1c), and fatty acid synthase (FAS). It also induced the expression of phosphorylation of AMP-activated protein kinase (AMPK). Conclusion: Pheophorbide A isolated from Gelidium amansii inhibit adipogenesis by down-regulating adipogenic transcription factors in 3T3-L1 adipocytes. These results suggest that pheophorbide A may be useful for the prevention or treatment of obesity owing to its inhibitory effect on adipogenesis.

Author(s): Ji Sook Han

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