Isolation, purification and characterization of alkaline pectinase from Bacillus subtilis isolated from soil

Pectinase is an enzyme that breaks down pectin, a polysaccharide found in plant cell walls. Commonly referred to as pectic enzymes. Pectinase producing microorganisms were isolated from pectin rich sites using selective isolation techniques and were screened for their pectinolytic activity. The culture conditions like pH, temperature, carbon sources and nitrogen sources were optimized. The culture conditions were optimized for maximum enzyme production by isolate microorganisms present in soil to produce large quantity of pectinase the fruit extract saturate in a beaker containing soil for 7 days. The screening and isolation of bacteria were done by various biochemical tests. The purification of pectinase enzymes were done by dialysis and Ion exchange Chromatography.

Author(s): Akhilesh Kushwaha

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