Isolation of Lichen Forming Fungus of Everniastrum cirrhatum and Evaluate its Antagonistic and Antimicrobial Activity

Mycobiont is a lichen forming fungi (LFF) symbiotically associated with phycobiont (algae) of lichens. Here LFF was isolated from Everniastrum cirrhatum lichen and checked its antagonist and antimicrobial properties against pathogens with minimum inhibitory concentration (MIC). The isolated LFF was inhibited the growth of several plant pathogens viz, Fusarium moniliforme, F. oxysporum and F. udum and Human pathogenic fungi viz, Epidermophyton floccosum, Microsporum gypseum and Trichophyton rubrum as well as pathogenic bacteria viz, Streptococcus mutant, Staphylococcus aureus, Salmonella typhimurium and Salmonella typhi. The antagonistic activity of LFF was found most effective against Fusarium udum and caused 55.55% inhibition of mycelial growth and antimicrobial substances from LFF were also compared with natural thallus extract, maximum activity was observed by acetone extracts of LFF against Microsporum gypseum (21mm) and its MIC was found to be 0.78125×10-7 μl against Microsporum gypseum and Candida albicans. Therefore acetone extracts of LFF found most effective inhibitor rather than other extracts. This is the 1st attempt to evaluate antagonistic and antimicrobial properties of LFF (Everniastrum cirrhatum) against pathogenic fungi and bacteria.

Author(s): Shaily Javeria*, Sushil Kumar Shahi, Mamta Patra Shahi, Yashwant Kumar Yadava

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