Isolation and Identification of Proteins in the Flow Sap during Grape Germinating Stage

The sap flowed abundantly in the tree when grape recovered to growth in spring. The flow sap delivered many kinds of nutrient substances for the grape growth. To reveal the importance of the sap during grape development the proteins from the flow sap were collected from ‘Rosario Bianco’ at different developmental stage, and then analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional gel electrophoresis (2-DE). We found the protein of flow sap was annotated as peroxidases, chitinase, glycosyl hydrolase and α-Larabinofuranosidase, which play an important role at the development and growth of grape. However, there are some functions of proteins still are unknown. The unknown function group consisted of six proteins. This study will help to further explain the important role of flow sap in grape growth and development

Author(s): Zhenqiang Xie, Ting Zheng, Xudong Zhu, Xiangpeng Leng, Haifeng Jia, Jinggui Fang

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