Inhibitory effects of calophyllum inophyllum extract on atopic dermatitis induced by DNCB in mouse

Objective: Atopic dermatitis, or eczema, is a common skin disease that isoften associated with other atopic disorders, such as allergic rhinitis and asthma. One hypotheses concerning the mechanism of atopic dermatitis that the primary defect resides in an immunologic disturbance thatcauses IgE-mediated sensitization, with epithelialbarrier dysfunction regarded as aconsequence of the local inflammation. Calophylluminophyllumextract(CIE) has been used in oriental medicines and many previous studies reported its antimicrobial and anti-inflammatory effect via cyclooxygenase inhibitions. This study examined whether the CIE exerting anti inflammation could alleviate the symptoms of atopicdermatitis (AD) induced with 2,4-dinitrochlorobenzene(DNCB) in mice.
Methods: Mice were sensitized and challenged on the skin of their backs with DNCB. At 28-54 days after sensitization, mice were treated with topical application of CIE as skin ointment. Skin thickness, collagen density, serum cytokine analysis and scratching behavior test were used to evaluate the effects of CIE on DNCBinduced mice by ELISA, histological analysis.
Results: Topical application of CIE attenuated AD-like skin inflammatory symptoms. CIE decreased scratching frequencies, the epidermal thickness, fibrotic tissue changeof dorsal skinand the level of inflammatory cytokines-IgE and histamine.
Conclusion: CIE properly improved AD-like skin inflammatory symptoms on the dorsal skin of DNCB-inducedmice, partly by suppressing production of histamine and IgE, fibrotic changes in inflamed skin. Therefore, CIE is a potential therapeutic agent for skin inflammatory diseases such as AD.

Author(s): Yu Lim Um and Young Wook Jo

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