In vivo Study of Aqueous Extract of Zingiber officinale in Modulating DMBA Induced Genotoxicity in Albino Rats

Objective: To study the anti-genotoxic properties of aqueous Ginger extract on Dimethyl benz (a) anthracene induced genotoxicity in rat bone marrow cells.
Methods: Group I (Normal control) animals were administered with distilled water (DW). Group III and IV animals were administered with 250mg/kg and 500 mg/kg aqueous extract of Zingiber officinale (ZE) orally, for seven consecutive days. After twenty four hours of the last dose of test drug, Group II, III and IV rats were injected with Dimethyl benz (a) anthracene (DMBA) 30 mg/kg b.w. intraperitoneally. Group V and VI animals were kept with standard diet and 250mg/kg and 500mg/kg aqueous extract of Zingiber officinale (ZE) alone respectively. After twenty four hours of DMBA treatment, to arrest metaphase inject Colchicine (2 mM) 3 mg/kg body weight intraperitoneally before the animals were sacrificed.
Results: Scoring of micronuclei in polychromatic (P) and normochromatic (N) erythrocytes followed by chromosomal aberrations were performed in bone marrow cells of animal. Both 250mg/kg and 500mg/kg body weight of Ginger extract produced a significant inhibition of DMBA induced genotoxicity like ratio of polychromatic to normochromatic (P/N) and frequency of micronuclei. It decreased the total chromosomal aberrations significantly, the DMBA induced chromosomal breaks, gaps, rings, deletions and other abnormalities in bone marrow cells were inhibited. Other biochemical parameters like plasma MDA, SOD and liver MDA, SOD and GSH were towards normal in DMBA injected rats in a significant extent (p<0.001).
Conclusion: Aqueous extract of Zingiber officinale has antigenotoxic property against DMBA induced genotoxicity.

Author(s): Sradhasini Rout, Anjan Kumar and Bandana Rath

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