In vitro study of plant extract from Chenopodium album that inhibits a key enzyme in diabetes and its role in diabetic oxidative stress

Chenopodium album (CA) is traditional Indian herbal medicinal plant. It is used widely all over India and other parts of the world. The plant is traditionally used as antidiabetic, laxative, anthelmintic and cardio-tonic, snake bite, skin disease etc. In this study, in vitro antidiabetic activity antioxidant activity, phenolic content and flavonoid content of different extracts of C. album were determined. Antioxidant study was carried out by using different models i.e. DPPH scavenging, H₂O₂ free radicals, reducing power and phosphomolybdenum assay. In vitro antidiabetic activity was evaluated by using α-amylase inhibition assay. In the DPPH radical scavenging results are obtained in the following order: Methanolic leaves >ethyl acetate roots >methanolic leaves > ethyl acetate roots extract. From reducing power, the position of arrangement for reducing power was methanol root >methanol leaves>ethyl acetate root>ethyl acetate leaves. In the hydrogen peroxide activity of extracts from Chenopodium album was in the order of methanolic leaves extract > ethyl acetate leaves extract >ethyl acetate roots extract >methanol roots extracts respectively. From the phosphomolybdate method, antioxidant capacity of ethyl acetate root extracts of Chenopodium album was found be maximum.

Author(s): Pushpander Kumar , Sunil Kumar , Suresh Kumar and Ravindra Kumar

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