In vitro shoot multiplication in different species of Banana

The present investigation was undertaken to study the effect of different concentrations of BAP on shooting in different species of Banana viz. Ardhapuri, Basrai, Shrimanti. Sword suckers with medium size were inoculated on M. S. medium supplemented with different concentrations of BAP (3mg/l, 5mg/l, 7mg/l, 9mg/l) cultures were incubated at 25+10 C with a 16 hr photoperiod (2000 lux) provided by cool white florescent tubes. The pH of medium was adjusted to 5.8 prior to autoclaving. The materials were sub-cultured at 30 day interval in same medium to produce multiple shoots. The effect of different concentration of regimes of BAP on bud initiation and shoot multiplication were investigated.

Author(s): U. P. Bhosale *, S. V. Dubhashi , N. S. Mali and H. P. Rathod .

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