In vitro plant regeneration using adventitious roots as explants in Tylophora indica

In vitro regeneration of plants using adventitious roots as explants in T. indica has been studied. In the present study, various auxins such as 2,4-dichlorophenoxyacetic acid (2,4-D), indole butyric acid (IBA) and naphthalene acetic acid (NAA) alone were tested at different concentrations on Murashige and Skoog (MS) medium with 3% sucrose. Maximum shoot regeneration (25.0) per treatment was achieved when adventitious roots were cultured with IBA at 2.0 mg/L concentration. Shoot bud elongation was achieved on MS full strength with BAP at 0.1 mg/L and rooting was on MS half strength medium with IAA at 0.2 mg/L concentration. Plants were established in a potting substrate. The present protocol will serve as alternative mean for in vitro clonal propagation of this endangered plant of immense medicinal value.

Author(s): Abdul Nayeem*, Rashmi M. Panchakshararadhya and Vedamurthy A. Basappa

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