In vitro micropropagation and antimicrobial activity of Solanum trilobatum

Solanum trilobatum is one of the oldest known therapeutic herbs belonging to the family solanaceae. To establish a suitable protocol for micropropagation in S. trilobatum leaf and node explants were used. The explants were surface sterilized with 1% Bavistin and 0.1% Mercuric chloride for 3min and it was found to be satisfactory in controlling the contamination. MS medium supplemented with BAP 2.0mg/l and Kinetin 2.0mg/l was found to initiate the shoot from node explants with in10-15days. The root induction was obtained in MS media containing 2, 4-D (2.0mg/l) and IBA (2.0mg/l). For callus initiation node and leaf explants are used, NAA + Kinetin (2.0+2.0mg/l) was found good combination for induction of callus from node, NAA + Kinetin(2.0+2.0mg/l) and NAA + BAP (2.0+2.0mg/l) was found good combination for induction of callus for leaf explants. The n-butanol extract of S. trilobatum showed highly effective against K. pneumoniae, B. cereus, S. boydii, S. aureus. There is no comparative study of in vivo and in vitro plants activity. In this study we found that in vitro grown plants showed better activity when compare to in vivo plants.

Author(s): S.R.Sathish Kumar, K.M.Sakthivel, L.Karthik, S.Mythili, A.Sathiavelu*

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