In vitro investigation on effect of elicitors and precursors on the synthesis of anti-Parkinsonâ??s drug (L-DOPA) from Mucuna imbricata

The bioprocess methodology for the enhanced production of phytochemicals using plant cultures predominantly depends on the elicitors and precursors. The drug L-DOPA (anti-Parkinson’s drug)(L-3,4-dihydroxyphenylalanine) has been isolated from Mucuna plant which is a major source of this drug. In the present study, effects of different elicitors and precursors on in vitro grown callus in the optimized medium were carried out to enhance the accumulation of L-DOPA and other secondary metabolites production. Results revealed that positive influence of Methyl Jasmonate and Salicylic Acid on the accumulation of L-DOPA and other secondary metabolites (Nutritional and anti-nutritional factors). Results also illustrated that a significant suppression callus biomass dry and moist weight as will as total phenolic, flavonoids and antioxidants content in the elicited callus from day 30 to day 45 of inoculation. Additionally, results clearly seem that improved accumulation of carbohydrates was positively correlated with an increase in L-DOPA content in the elicited callus. These methods boost the nutritional potency imparting additional health benefits. It is also concluded that the use of these techniques helps to make pure source of L-DOPA implemented in the prevention of Parkinson’s disease.

Author(s):  Suresh Suryawanshi1, Parthraj Kshirsagar2, Prajakta Kamble3, Vishwas Bapat4, Jyoti Jadhav1,4*

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