In vitro immunomodulatory study of different parts of Prunus cerasus L. (sour cherry) Plant

Five different parts (fruit, seed, root, leaf and stem-bark) of sour cherry Prunus cerasus L. were extracted with methanol, water-methanol 1:1 and water. Total fifteen extracts were prepared and screened for In vitro immunomodulatory potential employing Nitroblue Tetrazolium Reduction test (NBT), Inducible Nitric Oxide Synthase test iNOS), Bactericidal activity of macrophages (Phagocytosis) and T and B cell proliferation by MTT assay. Results showed a significant difference in the immumodulatory activities according to the parts of sour cherry plant. Among all tested extracts, the methanolic fruit extract (Pc-MeOH-F-Ext.) showed the maximum potential for NBT, iNOS and Phagocytosis as well as splenocyte proliferation, whereas the aqueous seed extract (Pc-W-S-Ext.) showed the minimum effect. Further a preliminary analysis of the most immunopotent extract (Pc-MeOH-F-Ext.) showed the presence of bioactive phytochemicals like flavonoids, anthocyanins, etc. The results of this study demonstrate the immunostimulatory effect of Prunus cerasus L. in a concentration-dependent manner. The results suggest the fruits of sour cherry could be applied as an immunomodulator.

Author(s): Sheikh Abid Alia, Aruna Bhatiab*, Qazi Parvaiza, H. M. Bhatc, Sheikh Fayaz Ahmadd, Nishu Kherab and Refaz Ahmada

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