In vitro Callus Regeneration and Biochemical Analysis in the Medicinal Plant Phyllanthus niruri L.

Objectives The main objective of the present study is to find out the callus initiation in the plant Phillanthus niruri and to determine its biochemistry. Method The callus formation was done with MS medium and its biochemical analysis was done with the standard methods 12 - 14. Result Callus growth was more in explants taken from apical bud and nodal segment nearer to the apical bud. But the callus growth was delayed in explants taken from mature leaves and nodal segments away from the apical bud. In the phytochemical analysis Amino acid, protein, total sugar, starch and phenol content was more in aerial part of the P. niruri. Conclusion The callus growth is more from apical bud than compared to matured leaves and nodal segments far from apical bud. The phytochemicals such as Amino acid, protein, total sugar, starch and phenol were more in apical parts than the matured portion.

Author(s): J Jerome Jeyakumar, M Kamaraj, S Srinivasan, V Anburaja and D Prema

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