In Vitro Assessment of the Antioxidant and Anti-Inflammatory Effects of Globularia Alypum L . Leaves

Objective

This work allowed the valorization of the leaves of Globularia alypum L. by extracting their global biochemical composition and evaluating their biological potential.

Methods

The extraction was based on the use of four different methods (soxhlet, sonication, maceration and infusion) in order to compare their effect on the quality of polyphenols and thus their potential efficacy to exploit in different domains.

Results

The highest composition of polyphenols values was evaluated by infusion and soxhlet extract respectively (89.35–74.25 mg Eq GAE/g ext. While, soxhlet method only assured an important extraction of flavonoids and flavonols (65.45 mg CAT/g ext and 44.16 mg RE/g ext.). The samples were an area under discussion to a screening for their antioxidant activities using the DPPH. FRAP and Nitric oxide assays. Soxhlet and maceration of leaves of G. alypum showed the important antioxidant activity (DPPH and Nitric oxide) with an IC50 (0.28; 0.34 mg/ml and 0.57; 0.67 mg/ml respectively). Concerning, the infusion and the maceration extract, it showed an important Radical-Scavenging Activity and Ferric Reducing (FRAP) with IC50 (0.34 and 0.38 mg/ml respectively). For the first time, the in vitro anti-inflammatory activity of the leaves G. alypum tested by four extraction methods was realized. The soxhlet and maceration methods revealed an important anti-inflammatory effect by stabilization membrane (1.45 and 2.01 mg/ml respectively) and inhibition of protein denaturation (albumin) (1.80 and 1.73 mg/ml respectively).

Conclusion: As a term of exploitation and valorisation, the leaves of G. alypum have antioxidant and anti-inflammatory effects thanks to their richness in phenols, flavonoids, flavonols, anthacynins, alkaloids, saponins and vitamin E.

Author(s): Nouir Sahar, Houda Haddad, Amani Khalifa and Amira Zari

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