In vitro antioxidant, anti-lipid peroxidation activities and HPLC analysis of methanol extracts from bark and stem of Mahonia leschenaultia takeda

The antioxidant activity, anti-lipid peroxidation activity, polyphenolic contents and HPLC analysis of bark and stem of Mahonia leschenaultia takeda was investigated. Total phenolic and flavonoid contents were determined using folin-ciocalteu reagent method and aluminium chloride method respectively. Antioxidant activity was evaluated by the following methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH.) quenching assay, reducing capacity, scavenging capacities towards hydroxyl ion radicals (.OH), nitric oxide (NO), Hydrogen peroxide (H2O2); iron chelating capacity, ferric reducing antioxidant power (FRAP) and anti-lipid peroxidation assays. The bark extract exhibited 5346.1 ± 1.040 mg GAE/100g phenolic content, 4317.2 ± 0.946 mg QE/100g flavonoid content and 5346.3 ± 0.720 mg QE/100g flavonol content. The stem extract afforded a phenolic content of 5255.8 ± 0.644 mg GAE/100 g, flavonoid content of 3082 ± 0.917 mg QE/100 g and flavonol content of 3363.3 ± 0.544 mg GAE/100 g. The methanolic extracts from bark (EC50 40 ± 1.34) and stem (EC50 50 ± 1.10) exhibited high scavenging capacity towards DPPH, nitric oxide and hydrogen peroxide. The reductive capacity and FRAP of the extracts increased with increasing concentration of the samples. The antioxidant capacities of the extracts were comparable with the antioxidant standards, butyl hydroxy toluene (BHT) and EDTA. Significant and positive correlations were observed between polyphenolic contents and antioxidant capacities, indicating that the phenolics were major contributors of the antioxidant property. The HPLC analysis of the extracts showed the presence of the alkaloid Berberine. The results of the present study revealed that the bark and stem of M. leschenaultia are the natural radical scavengers with potent antioxidant activity.

Author(s): Engoor Sugathakumar Adithya, Mangalanandan Santha Lakshmi, Paul Hephzibah Christabel and Jagathala Mahalingam Sasikumar

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