In vitro Antioxidant and Antibacterial Potential of Leaf and Stem of Gloriosa superba L.

In the present study, leaf and stem of Gloriosa superba L. were selected to evaluate its antioxidant and antibacterial potential. The extraction was done by a cold percolation method using solvents like hexane, ethyl acetate and methanol. The antioxidant activity was evaluated using antioxidant assays like superoxide radical scavenging assay, Ferric reducing antioxidant power and reducing capacity assessment. Total phenol and flavonoid content was measured by Folin- ciocalteu’s reagent and aluminium chloride colorimetric method respectively. The antibacterial activity was done by an agar well diffusion method against four Gram positive and four Gram negative bacteria. The methanol extract of leaf and stem showed highest FRAP activity and highest reducing capacity assessment respectively. There was a direct correlation between total phenolic content and antioxidant activity. Gram positive bacteria were more susceptible than Gram negative bacteria. Methanolic extract of leaf showed best antioxidant and antibacterial activity and can be furth

Author(s): Pooja Moteriya, Jalpa Ram, Tejas Rathod and Sumitra Chanda

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