Identification of new genomic sources for aluminum tolerance in hexaploid wheat breeding lines using phenotypic and genotypic screening

The present study was investigated in a set of 45 hexaploid
wheat breeding lines collected from South Africa,
Kenya, Egypt, Cameroon, Ethiopia and Australia for acid
soil with aluminum tolerance through phenotypic and
genotypic screening. Among these, Betta (acid soil-sensitive),
Atlas 66 (acid soil-tolerant) and Chinese spring
(partially tolerant) were included as standard controls
cultivars for phenotypic and genotypic analysis, while
four international standard check varieties (Carazinho,
Egret, ES8, ET8) were used when studying the allelic variations
of resistance gene (MATE1B). These accessions
were phenotyped in a greenhouse experiment and genotyped
through genotyping-by-sequencing (GBS). Standard
checks showed a citrate efflux concentration of 8.6
μmol (Atlas66), 7.66 μmol (Chinese spring) and 6.1 μmol
(Betta) and among the evaluated genotypes, 18 best lines
were selected for their high tolerance through phenotypic
screening. Furthermore, the GBS protocol used for characterizing
genetically accessions have produced 80 124
single nucleotide polymorphism (SNP) markers. So, we
defined SNP haplotypes around the MATE1B gene and
identified 4 markers defining three distinct haplotypes
around, where accessions have carried the alleles CATA
for haplotype A, CGCG for haplotype B, and TGCG for
haplotype C. For all traits, the phenotypes corresponding
to haplotype A were significantly highest than others. To
conclude, we suggest that SNP markers associated with
haplotype A will provide a useful tool in marker-assisted
breeding programs to improve wheat productivity in acid
soil under aluminum toxicity conditions.

Author(s): Honoré Tékeu , Willem C. Botes, Peter R. Ryan, Aurèlie Tardive, Eddy Ngonkeu, François P. Djocgoué, Martine Jean, François Belzile

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