HPTLC method development and validation for the estimation of Lopinavir and Ritonavir in capsule dosage form

A simple, selective, linear, precise and accurate HPTLC method was developed and validated for rapid assay of Lopinavir and Ritonavir in capsule dosage form. The separation was achieved on aluminum plate 60F254, (10 × 10 & 20 × 10 cm) with 250 μm thickness as the stationary phase and the mobile phase consisted of chloroform: methanol: ammonia (8:2:0.2, v/v). The solvent system was found to give compact spot for Lopinavir and Ritonavir (Rf values of 0.81 and 0.53 min). Densitometry analysis was carried out in the absorbance mode at 254 nm. The linear regression analysis data for the calibration plots showed good linear relationship with respect to peak area in the concentration range 100-600 μg and 100-700 μg of Lopinavir and Ritonavir (with r = 0.9999 and 0.9999). The method was validated for limit of detection, limit of quantitation, accuracy, precision, robustness and recovery. The result and statistical analysis proves that the developed method is reproducible and selective for the estimation of said drug. The proposed method can be successfully applied for the estimation of Lopinavir and Ritonavir in capsule dosage forms.

Author(s): Monali Bhalerao

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