HPTLC method development and validation for the estimation of Labatelol Hydrochloride in tablet dosage form

A simple, selective, linear, precise and accurate HPTLC method was developed and validated for rapid assay of Labatelol Hydrochloride in tablet dosage form. The separation was achieved on aluminum plate 60F254, (10 × 10 & 20 × 10 cm) with 250 μm thickness as the stationary phase and the mobile phase consisted of chloroform: methanol: ammonia (8:2:0.2, v/v). The solvent system was found to give compact spot for Labatelol Hydrochloride (Rf values of 0.49). Densitometric analysis was carried out in the absorbance mode at 254 nm. The linear regression analysis data for the calibration plots showed good linear relationship with respect to peak area in the concentration range 0.50-1.50 μg spot-1 of Labatelol Hydrochloride (with r = 0.99774). The method was validated for limit of detection, limit of quantitation, accuracy, precision, robustness and recovery. The result and statistical analysis proves that the developed method is reproducible and selective for the estimation of said drug. The proposed method can be successfully applied for the estimation of Labatelol Hydrochloride in tablet dosage forms.

Author(s): Monali R. Bhalerao

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