HPTLC Analysis of Mentha piperita and Citrus sinensis

Mentha piperita and Citrus sinensis was an aromatic plants well known for its fragrance and distinct aroma. In this prospective study to evaluate the chromatogram detection of ethanol extract of Mentha piperita and Citrus sinensis with standard flavonoid marker eriocitrin by HPTLC technique. HPTLC chromatogram was developed in ethanolic extract of Mentha piperita, Citrus sinensis and standard flavonoid marker eriocitrin by using Toluene-Ethyl acetate-Formic acid-Methanol (3:6:1.6:0.4) as mobile phase. The identified bands of compounds 1-9 in the ethanolic extracts of Mentha piperita and Citrus sinensis was confirmed under the TLC scanner with the standard marker eriocitrin at 254nm.

Author(s): S.K. Gangai Abirami and P. Nirmala

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