HPLC-DAD Fingerprinting of Ethanol Extracts from Conyza sumatrensis and Spathodea campanulata and their Additive Effect in Plasmodium berghei K173 Infected Mice

The aim of the present study was to develop an appropriate HPLC method to identify different constituents from Conyza sumatrensis (CS) and Spathodea campanulata (SC) and evaluate antimalarial potential of the extract combination with reference to the traditional use. Schizontocidal activity was measured using a standard in vivo assay, with Plasmodium berghei K173, a chloroquine sensitive strain. The combination of SC and CS ethanol extracts gave statistically significant and improved suppressions which ranged from 83 to 99.01%. Remarkable suppression in parasitaemia resulted into increased mean survival time relative to vehicle control. The HPLC fingerprints of SC and CS extracts showed numerous peaks. Moreover, the same for the combination revealed 23 peaks among which gallic acid, catechol, coumarin and quercetin peaks could be detected. These compounds may be responsible for the activity of the extract towards Plasmodium berghei K173 infected mice. Therefore, SC and CS warrants further evaluation in the search for novel antimalarial drugs.

Author(s): Pone Kamdem Boniface,  Manju Singh and Anirban Pal

Abstract | PDF

Share This Article