HPLC method development of rivastigmine by RP-HPLC in its bulk dosage form

A fast simple sensitive precise, accurate and reproducible RP-HPLC method was developed and validated for the analysis of rivastigmine bulk dosages form. The separation was conducted by using C-18 RP-HPLC column. Which was maintained at ambient temperature. The mobile phase consist Potassium dihydrogen phosphate buffer and acetonitrile (70/30 v/v) was delivered at a rate of 1ml/min. The analysis was detected by using UV detector at the wavelength217nm.The method is validated for its precision, limit of quantitiation (LOQ) linearity and robustness. The method was found to be linear over the concentration range 10-100 μg/ml (r² =0.999). The retention time for rivastigmine was found to be 3.66±25min. limit of quantitation of method is 0.196μ/ml and limit of detection 0.056μ/ml.

Author(s): S. Navaneethakrishnan, MD. Reshma Begum, P. Suresh Kumar, J. Belson David and M. Phani Kumar

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