Hepatoprotective Potential of Ziziphus oenoplia (L.) Mill Roots against Paracetamol- Induced Hepatotoxicity in Rats

Objective: To evaluate the hepatoprotective potential of ethyl acetate fraction of Ziziphus oenoplia (L.) Mill roots against paracetamol induced hepatotoxicity in Wistar albino rats.
Materials and Methods: Paracetamol-treated groups (II, III, IV and V) were administered orally single daily dose of fraction (150 and 300 mg/kg) for 14 days. The serum levels of glutamic oxaloacetic transaminase (SGOT), glutamate pyruvate transaminase (SGPT), alkaline phosphatase (SALP), γ-glutamyltransferase (γ -GT), and bilirubin were analyzed together with glutathione S-transferase (GST), glutathione reductase (GR), hepatic malondialdehyde formation and glutathione content.
Results and Discussion: The significantly high activity of serum enzymes, SGOT, SGPT, SALP, γ-GT, and bilirubin due to paracetamol, were regained to normal in a dose-dependent manner. Meanwhile the reduced activities of GST and GR were also regained to normal. Additionally, ethyl acetate fraction also extremely interrupted the increase of hepatic malondialdehyde formation and decrease of reduced glutathione content in the liver of paracetamolintoxicated rats in a dose-dependent manner. Silymarin also showed well hepatoprotective activity on post-treatment against paracetamolinduced hepatotoxicity in rats. The biochemical observations play as supplementary component with histopathological assessment of rat liver sections. It is concluded that ethyl acetate fraction has an effective hepatoprotective action against paracetamol-induced hepatic injure in rats.

Author(s): Arpita Singh, G. Rajesh Kumar, Shyam Sundar Gupta, Satyawan Singh, A.K.S. Rawat and Ch. V. Rao

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