Hairy Root Culture: A Promising Approach in Biotransformation

The production of hairy root culture by soil bacterium Agrobacterium rhizogene mediated gene transformation using plant tissue culture. Hairy roots induced when explants of plant infected with root inducing plasmid of Agrobacterium rhizogenes, T- DNA transmit in plant cell and express along with plant genome. Hairy root culture is novel source for valuable secondary metabolite production because of its fast growth without hormones, biochemical and genetic stability. To prevent destruction of root harvesting from several important plant species hence production of large amount hairy roots through in-vitro condition. The different strains are responsible for construction of hairy roots. This has been demoralized to make use of hairy roots as a convenient and superior option to yield constructive metabolites from the plants. Numerous compensation from using the hairy roots as a supply for plant products as well as facility for modification of metabolic pathways by integration of related genes have been demonstrated in recent times in plants of diverse origin. Integration of desirable genes to the plants using Agrobacterium rhizogenes mediated genetic transformation for plant improvement has also been successfully accomplished in several agriculture horticulture crops and in woody plants.

Author(s): Vrishali V Korde, Snehal S Dhas and Neha A Gurave

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